September 14 is Florida Missing Children's Day, on which families, law enforcement officers and citizens gather to remember children who are still missing and those who will never come home again.

According to the National Center for Missing and Exploited Children, there are 327 unsolved missing children's cases in Florida alone.

One of the most famous unsolved cases in Central Florida surrounds of 2-year-old Trenton Duckett, who disappeared from Leesburg nine years ago.

"He was just a bundle of joy. He was a lot of fun to be around," said Trenton's father, Josh Duckett. "It's hard for me to look at the pictures and see him as an 11-year-old."

Trenton was last seen on Aug. 27, 2006. Vanished from his mother's home without a trace and without signs of a struggle, suspicion pointed to the 2-year-old's parents, Josh and Melinda Duckett, who were going through a bitter divorce and custody battle at the time.

"She had reported him missing, and she had told them that I took him," Josh Duckett said. "Almost 18 hours I went through questioning."

While Josh cooperated with the investigation, evidence started to stack up against the mother's story, including a fake email Melinda Duckett shared with Leesburg police, claiming her husband threatened to hurt her and Trenton.

"She logged into his account," said Leesburg police Maj. Steve Rockefeller.

Suspecting Melissa Duckett was trying to frame her husband, investigators took a closer look at Trenton's bedroom, where Melinda said her son was taken through a cut in the window screen.

"There was no disturbance on the ground outside of the window," Rockefeller said. "It just didn't match with somebody taking the child."

Melinda Duckett was studying criminal psychology, and at the same time was being investigated for embezzling thousands of dollars from the bank where she worked.

"Melinda was the only suspect in the case," Josh Duckett said.

But there was still no sign or scent of 2-year-old Trenton.

"We were trying to follow her and talk to her," Rockefeller said.

Almost two weeks after Trenton disappeared, Melinda Duckett agreed to appear on TV for an interview with Nancy Grace.

"She asked questions that legitimately needed to be answered," Josh Duckett said.

But that interview didn't go as Melinda had planned. Police found notes on her computer with prepared questions and answers.

"She wasn't being portrayed as the victim she wanted to be," Rockefeller said.

Soon after the interview, Melinda killed herself with a shotgun. Leesburg police lost their only suspect

"I really do think that she just killed herself because she was no longer the victim, and she still just didn't want Josh to have him," Rockefeller said.

"We're basically at a dead standstill," Josh Duckett said. "We're nine years into it, and we're no closer to getting any kind of answers than we were back in 2006."

Today, Josh Duckett continues to host candlelight vigils for Trenton and raise awareness for other missing children.

"There are hundreds and hundreds of them," he said. "If we bring one child home, it's a success."

Leesburg police said Trenton's case remains active and open, yet, Josh Duckett said he hopes another investigating unit will take a look at the case in hopes of one day bringing Trenton home.

"The hard part is not knowing," he said.

Missing Children's Day in Tallahassee

Parents of missing children from across the state visited Tallahassee on Monday to mark Florida Missing Children's Day.

The families meet there every year to pray together that their children will someday be found.

One of their top priorities this year was to lobby lawmakers for more DNA testing in cases. Critics say state crime lab funding is not what it should be, and collecting DNA samples found at crime scenes with the DNA of the missing can sometimes uncover critical leads.

During Monday's ceremony, parents urged lawmakers to come up with more money in next year's budget.